Extraction, Purification and Therapeutic Use of Bacteriophage Endolysin against Multi-Drug Resistant Staphylococcus aureus: in-vivo and in-vitro study
Year: 2018
Type: article
Abstract: Background Resistant infection with multidrug-resistant Staphylococcus aureus representing a real problem for health care providers.Bacteriophage lytic enzymes or lysins are highly evolved molecules that have been specifically developed by phages to quickly and efficiently allow their progeny to be released from the host bacterium while destructing that bacterium.Objectives Isolation of endolysin from S. aureus bacteriophages, and administering them systemic in vivo lab animal and measure the therapeutic efficacy, as well as evaluation of their biosafety.Method This study was performed from March 2015 to August 2017, 50 bacteriological samples of S. aureus were collected, and examined with their anti-biogram, then bacteriophage cocktails were done for five resistant strains of them.Endolysins were extracted from their corresponding bacteriophages, they were characterized and the enzymatic and antibacterial activities assays were performed on them in addition to in vivo trial on animals regarding the therapeutic effect of these extracted endolysins on laboratory mice. ResultsThis study showed that the extracted endolysin from these bacteriophages was effective in treating laboratory mice from bacteremia with S. aureus and saving their lives when injected intraperitoneal.Conclusion Endolysin can be extracted directly from their bacteriophages and used by injection of mice with bacteremia with the proper dose of the extracted endolysin of the corresponding bacteriophages which was effective in all of them.
Institutions: Nahrain University, Mustansiriyah University
Language: English
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $300
OpenAlex ID: W2796154591