Work

Double-layered microsphere based dual growth factor delivery system for guided bone regeneration

Year: 2018

Type: article

Abstract: release testing showed that bioactivity of both growth factors retained within the microspheres and differential release of SDF-1 and BMP-2 was achieved. Microspheres with both growth factors showed an obvious chemotaxis effect on preosteoblasts by inducing more cell migration. In osteoinductive ability tests, the microspheres with both growth factors showed higher ALP activity and more mineralized modules than control groups after culturing for 2 weeks. The expression of bone development transcription factors (Runx2, OCN, Osterix) as well as Smad signals (Smad 1, 5, 8) showed higher gene expression in the dual growth factor group. Our results suggest that a double-layered microsphere system enhances the recruitment of osteogenic cells and osteoinduction, which provides a promising platform for bone regeneration.

Source: RSC Advances

DOI: 10.1039/c8ra02072h