Year: 2018
Type: article
Abstract: Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm⁻egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9/CD81 double null female mice. In this study, the putative mechanism of CD9/CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9/CD81 localization visualised in detail by super-resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosome reaction in human sperm, only CD9 is relocated, compared to the relocation of both proteins in mice. The structural modelling of CD9 and CD81 homologous and possibly heterologous network formation was used to propose their lateral Cis as well as Trans interactions within the sperm membrane and during sperm⁻egg membrane fusion.
DOI: 10.3390/ijms19041236
Institutions: Czech Academy of Sciences, Czech Academy of Sciences, Institute of Biotechnology, Czech University of Life Sciences Prague, Slovak Academy of Sciences, Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Biochemistry and Genetics of the Slovak Academy of Sciences, Charles University
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,499
Awards: G11386687, G12500569, G1342419356, G1376288820, G1507827888, G1735756308, G176604203, G2259931850, G2784031255, G291036901, G321106277, G3364881555, G3490171961, G3691977473, G3803821774, G4131035492, G4280972364, G4410300904, G4433258970, G4470331596, G4626930849, G5466071070, G5479454656, G5490493097, G5916014575, Monitoring of protein network dynamics during sperm-egg membrane interaction, G6545326099, G6630972494, Etablovanie techník kryouchovania ovariálného tkaniva hovädzieho dobytka pre účely génovej banky, G777586394, G791075710, G8003743317, G8029139161, G803096421, G8265045674, Prítomnosť tetraspanínov a partnerských molekúl v rozmnožovacej sústave hovädzieho dobytka a ich účasť v interakcii gamét., G8704714967
OpenAlex ID: W2801414873