Detection of Sub-fM DNA with Target Recycling and Self-Assembly Amplification on Graphene Field-Effect Biosensors
Year: 2018
Type: article
Abstract: All-electronic DNA biosensors based on graphene field-effect transistors (GFETs) offer the prospect of simple and cost-effective diagnostics. For GFET sensors based on complementary probe DNA, the sensitivity is limited by the binding affinity of the target oligonucleotide, in the nM range for 20 mer targets. We report a ∼20 000× improvement in sensitivity through the use of engineered hairpin probe DNA that allows for target recycling and hybridization chain reaction. This enables detection of 21 mer target DNA at sub-fM concentration and provides superior specificity against single-base mismatched oligomers. The work is based on a scalable fabrication process for biosensor arrays that is suitable for multiplexed detection. This approach overcomes the binding-affinity-dependent sensitivity of nucleic acid biosensors and offers a pathway toward multiplexed and label-free nucleic acid testing with high accuracy and selectivity.
Source: Nano Letters
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,759
Funders: National Science Foundation, Bill and Melinda Gates Foundation, University of Pennsylvania, Pennsylvania State University, Ministry of Science and Technology of the People's Republic of China, Research Grants Council, University Grants Committee, Innovation and Technology Commission, Guangzhou Science and Technology Program key projects, National Key Research and Development Program of China, Division of Emerging Frontiers in Research and Innovation
OpenAlex ID: W2803554466