High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
Year: 2018
Type: article
Abstract: Method (O2M). O2M uses patterns from chemical-genetic datasets to identify mutants that are hypersensitive to each molecule in a synergistic pair but not to other molecules. The Brown lab exploits this growth difference by performing a high-throughput screen for molecules that inhibit the growth of mutant but not wild-type cells. The lab's work previously identified molecules that synergize with the antibiotic trimethoprim and the antifungal drug fluconazole using this strategy. Here, the authors present a method to screen for novel synergistic combinations, which can be altered for multiple microorganisms.
DOI: 10.3791/57241
Authors: Morgan A. Wambaugh, Jessica C. S. Brown
Institution: University of Utah
Language: English
Subfield: Drug Discovery
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2803723720