Measurement of apixaban concentrations in a real-world clinical and laboratory setting: the first Polish experience
Year: 2018
Type: article
Study design: Observational Study
Abstract: Materials and methodWe prospectively recruited the first 42 patients from John Paul II Hospital in Kraków, Poland, who received apixaban, including 10 individuals in whom the samples were drawn twice (a total of 52 samples).These patients were compared with 37 age-and sex--matched patients on rivaroxaban.Indications to measure the drug levels were doubts regarding compliance, bleeding complications, or assessment prior to invasive procedures.The PT (Thromborel S; reference range, 10.4-13.0s), aPTT (Pathromtin SL; reference range, 25.9-36.6s), and dRVVT assays using LA1 Screening Reagent for screening tests (LA1) and LA2 Confirmation Reagent for confirmatory tests (LA2) were performed on the BCS -XP automated analyzer (Siemens Healthcare, Marburg, Germany).Positive LA was defined as the LA1 -to -LA2 ratio exceeding 1.2.Apixaban and rivaroxaban concentrations were measured using the chromogenic Biophen DiXa I test (Hyphen, BioMed, Neuville--Sur -Oise, France) with specific calibrators.We adopted on -therapy ranges based on a summary of product characteristics 8 (for apixaban, 2.5 mg twice daily; peak, 30-153 ng/ml; trough, 11-90 ng/ml) and suggested by Douxfils et al 9 (for apixaban, 5 mg twice daily; peak, 59-302 ng/ml; trough, 22-177 ng/ml; for rivaroxaban, 20 mg once daily; peak, 189-419 ng/ml; trough, 6-87 ng/ml).Statistical analysis Continuous data were expressed as median (interquartile range [IQR]).Spearman's rank correlation analyses were used to estimate associations between variables.The Mann-Whitney test was used to compare differences between variables.The χ 2 test was used to compare categorical variables.A P value of less than 0.05 was considered significant.
DOI: 10.20452/pamw.4265
Institutions: John Paul II Hospital, Jagiellonian University
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W2804697322