Year: 2016
Type: article
Abstract: The amino acids supplemented to animal feedstuffs cannot be determined directly. Thus,we need to optimize the sample preparation step as well as the analytical method. In thisstudy, two hydrolysis mode were performed, one using 6 mol.L-1 hydrochloric acid at105°C for 24 h and the other based on microwave radiation-induced hydrolysis.Moreover, a chromatographic method was developed for simultaneous determination ofmethionine and lysine in feedstuffs, using conventional High Performance LiquidChromatography equipment equipped with an automated precolumn derivatizationsystem using ortho-phthalaldehyde (OPA) / mercapto-2-ethanol (ME) reagents and thefluorescence detector. Reversed-phase chromatography appears to be the preferredmethod, with a C18 stationary phase and a gradient elution. Doehlert matrix was used toget the optimal separation for 18 amino acids. System Suitability Test (SST), Linearity,Limit of Detection (LOD), Limit of Quantification (LOQ), Repeatability and Specificity ofthe method were studied. The LOD calculated were 93 and 9.4 pmol.μL-1 and the LOQwere 173.5 and 36.2 pmol.µL-1 for methionine and lysine, respectively.
Authors: Intissar Limam, Raouf Medimagh, Fayçal Hellal
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2804920120