Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites
Year: 2018
Type: article
Abstract: Membrane contact sites are cellular structures that mediate interorganelle exchange and communication.The two major tether proteins of the endoplasmic reticulum (ER), VAP-A and VAP-B, interact with proteins from other organelles that possess a small VAP-interacting motif, named FFAT [two phenylalanines (FF) in an acidic track (AT)].In this study, using an unbiased proteomic approach, we identify a novel ER tether named motile sperm domain-containing protein 2 (MOSPD2).We show that MOSPD2 possesses a Major Sperm Protein (MSP) domain which binds FFAT motifs and consequently allows membrane tethering in vitro.MOSPD2 is an ER-anchored protein, and it interacts with several FFAT-containing tether proteins from endosomes, mitochondria, or Golgi.Consequently, MOSPD2 and these organelle-bound proteins mediate the formation of contact sites between the ER and endosomes, mitochondria, or Golgi.Thus, we characterized here MOSPD2, a novel tethering component related to VAP proteins, bridging the ER with a variety of distinct organelles.
Source: EMBO Reports
Institutions: Centre National de la Recherche Scientifique, Inserm, Institut de génétique et de biologie moléculaire et cellulaire, Université de Strasbourg, Université Côte d'Azur, Institut de Pharmacologie Moléculaire et Cellulaire, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Biologie Intégrative de la Cellule, Hôpital d'Hautepierre
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $7,990
Funders: European Molecular Biology Laboratory, King's College London, Agence Nationale de la Recherche, Institut National de la Santé et de la Recherche Médicale, Universiteit Leiden, Université de Strasbourg, Ligue Contre le Cancer, Ministère de l'Enseignement Supérieur et de la Recherche, Centre National de la Recherche Scientifique, Leids Universitair Medisch Centrum, Institut National Du Cancer, French Infrastructure for Integrated Structural Biology
Awards: G1019071288, G1109023570, G1194865751, G1923432458, Par-delà les frontières, l'Université de Strasbourg, G2255650664, G2447855386, G2449524460, G2694206344, G3310169441, G3357951378, Molecular mechanisms of STARD3 addiction in HER2 tumors, G4460985483, G471331628, G4798114250, G4899307531, Integrative Biology : Nuclear dynamics- Regenerative medicine - Translational medicine, G7002111755, G7240651115, G7601735359, G7804020939, G8031520463, G8996902167
OpenAlex ID: W2806366302