Work

LCK as a Potential Therapeutic Target for Acute Rejection after Kidney Transplantation: A Bioinformatics Clue

Year: 2018

Type: article

Abstract: Objectives.We aim to identify the key biomarker of acute rejection (AR) after kidney transplantation via bioinformatics methods.Methods.The gene expression dataGSE75693of 30 samples with stable kidney transplantation recipients and 15 AR samples were downloaded and analyzed by the limma package to identify differentially expressed genes (DEGs). Then, Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were done to explore the biological functions and potential important pathways of DEGs. Finally, protein-protein interactions (PPIs) and literature mining were applied to construct the cocitation network and to select the hub protein.Results.A total of 437 upregulated genes and 353 downregulated genes were selected according to P<0.01 and log2fold change>1.0 . DEGs of AR are mainly located on membranes and impact the activation of receptors in immune responses. In the PPI network, Src kinase, lymphocyte kinase (LCK), CD3G, B2M, interferon-γ, CD3D, tumor necrosis factor, VAV1, and CD3E in the T cell receptor signaling pathway were selected as important factors, and LCK was identified as the hub protein.Conclusion.LCK, via acting on T-cell receptor, might be a potential therapeutic target for AR after kidney transplantation.

Source: Journal of Immunology Research

DOI: 10.1155/2018/6451298

Authors: Linpei Jia, Rufu Jia, Yinping Li, Xiaoxia Li, Qiang Jia, Hongliang Zhang

Institutions: Capital Medical University, Xuan Wu Hospital of the Capital Medical University, Cangzhou Central Hospital, National Natural Science Foundation of China

Language: English

Topic: Renal Transplantation Outcomes and Treatments

Subfield: Transplantation

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,830