Year: 2018
Type: article
Abstract: Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34+umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.
Source: Scientific Reports
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: GENERATION OF CORD BLOOD-DERIVED INDUCED PLURIPOTENT STEM CELLS USING A NON-VIRAL REPROGRAMMING STRATEGY, G1516346500, G1577006909, G1578237752, G1763632427, G196013823, Analysis of Cellular Heterogeneity for high resolution understanding of cancer, G212930404, G3840324753, CNC. IBILI, G5224927334, Functional Priming of Human Cord Blood Derived Haematopoietic Stem and Progenitor Cells, G7729689701, Indução de células estaminais pluripotentes a partir de células do sangue do cordão umbilical através de metodologia não-viral e a sua diferenciação em cardiomiócitos - iPSCardio, G8181744698, G8952488736
OpenAlex ID: W2807380229