Year: 2018
Type: article
Abstract: ABSTRACT The canonical role of cohesin is to mediate sister chromatid cohesion. In addition, cohesin plays important roles in processes such as DNA repair and regulation of gene expression. Mounting evidence suggests that various post-translational modifications, including phosphorylation, acetylation and sumoylation regulate cohesin functions. Our mass spectrometry analysis of cohesin purified from Schizosaccharomyces pombe cells revealed that the cohesin subunit Psm1 is methylated on two evolutionarily conserved lysine residues, K536 and K1200. We found that mutations that prevent methylation of Psm1 K536 and K1200 render sensitivity to DNA-damaging agents and show positive genetic interactions with mutations in genes encoding the Mus81–Eme1 endonuclease. Yeast two-hybrid and co-immunoprecipitation assays showed that there were interactions between subunits of the cohesin and Mus81–Eme1 complexes. We conclude that cohesin is methylated and that mutations that prevent methylation of Psm1 K536 and K1200 show synthetic phenotypes with mutants defective in the homologous recombination DNA repair pathway.
Source: Journal of Cell Science
DOI: 10.1242/jcs.214924
Institutions: University of Vienna, Max Perutz Labs, Comenius University Bratislava, Slovak Academy of Sciences, Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Biochemistry and Genetics of the Slovak Academy of Sciences, Cancer Research Institute of the Slovak Academy of Sciences, Biomedical Research Center of the Slovak Academy of Sciences, Research Institute of Molecular Pathology, Vienna Biocenter, Vienna BioCenter Core Facilities
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: Úloha proteínkináz v procesoch zúčastnených udržiavania stability genómu, Úloha esenciálnych proteínkináz v regulácii segregácie chromozómov počas meiózy, Bližšia charakterizácia a vylepšenie systému indukcie synchrónnej meiózy pri optimálnej teplote, Objasnenie mechanizmov posttranslačnej regulácie faktorov zostrihu RNA pri udržiavaní stability genómu, G2791369932, G2818153308, Charakterizácia nových mutantov s poruchou segregácie chromozómov počas meiózy, G2847787993, Chromosome dynamics, Charakterizácia nových otvorených čítacích rámcov v kvasinke Schizosaccharomyces pombe, G4556764370, Charakterizácia funkcií kohezínu v meióze, Chromosome segregation during meiosis, Vollantrag zu Chromosome Dynamics, The role of novel post-translational modifications of the cohesin, G8496149605, G8829974655
OpenAlex ID: W2807876443