Year: 2018
Type: article
Abstract: Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors sequentially or simultaneously. Collectively, a simple and rapid method was presented to knock out any DNA element conditionally. This approach will facilitate the functional studies of essential genes and regulatory regions during development.
Source: Stem Cells International
DOI: 10.1155/2018/9576959
Authors: Liangliang Chen, Ying Ye, Hongxia Dai, Heyao Zhang, Xue Zhang, Qiang Wu, Zhexin Zhu, Rapolas Spalinskas, Wenyan Ren, Wensheng Zhang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,570
Award: G3176339953
OpenAlex ID: W2808075666