Work

The aerolysin nanopore: from peptidomic to genomic applications

Year: 2018

Type: review

Abstract: The aerolysin pore (ARP) is a newly emerging nanopore that has been extensively used for peptide and protein sensing. Recently, several groups have explored the application of ARP in detecting genetic and epigenetic markers. This brief review summarizes the current applications of ARP, progressing from peptidomic to genomic detection; the recently reported site-directed mutagenesis of ARP; and new genomic DNA sensing approaches, and their advantages and disadvantages. This review will also discuss the perspectives and future applications of ARP for nucleic acid sequencing and biomolecule sensing.

Source: Nanoscale

DOI: 10.1039/c8nr04255a

Authors: Yong Wang, Li-Qun Gu, Kai Tian

Institutions: Arizona State University, University of Missouri, Columbia University

Language: English

Topic: Nanopore and Nanochannel Transport Studies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: Oxford Nanopore Technologies, National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences

Awards: Nanocross-nanopore for the detection of nucleic acid sequence and structure, Nanopore single-molecule dielectrophoresis and biomedical detection