Heterologous specific antiserum for identification of human T lymphocytes.
Year: 1974
Type: article
Abstract: Peripheral blood lymphocytes (PBL) from two patients with Bruton-type agammaglobulinaemia were used for the preparation of heterologous anti-human T cell-sera which were absorbed with cultured B lymphoblast cells, peripheral blood lymphocytes from a patient with chronic lymphatic leukaemia and `adherent' cells. Using multiple criteria, one antiserum (ATCS) was shown to be specific for T lymphocytes. This antiserum asserts the existence of human-specific T-lymphocyte antigen(s) (HTLA) and provides another method for identifying human T cells. In the presence of rabbit complement, ATCS was cytotoxic for 65·5% (range 49–78) of normal PBL and 97% of thymocytes (the latter cells having also a higher surface density of HTLA than PBL). The study of PBL from a variety of patients showed that the percentage of ATCS-sensitive cells was high in Bruton-type agammaglobulinaemia, variable from patient to patient and from time to time in common variable hypogammaglobulinaemia and generally low in active lepromatous leprosy, in patients under antilymphocyte globulin therapy and in chronic lymphatic leukaemia. Cultured lymphoblasts from various B cell lines or from a Burkitt lymphoma cell line were resistant to ATCS.
Source: PubMed Central
Authors: J L Touraine, F Touraine, D F Kiszkiss, Y S Choi, R A Good
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W28313187