Work

Extraction and characterization of biocolors from bacterial isolates of Pseudomonas sp. M1 and MS2

Year: 2018

Type: article

Abstract: In the current study, two pigments, i.e., yellow (M1) and orange (MS2) were extracted from Pseu domona s sp, isolated from hot water spring of Hima chal Pradesh.For the extraction, different solvents were used and maximum extraction was obtained in methanol followed by acetone.Extracted pigments showed  max at 508 nm (M1) and 459 nm (MS2), indicating that both the pigments are carotenoid.Thin Layer Chromatographic (TLC) analysis showed R f values of 0.90 and 0.91 for M1 and 0.75 and 0.18 for MS2.Spectral data of TLC fractions revealed that there are three fractions present such as β-carotene, torulene and torularhodin, respectively.The M1 and MS2 yielded stable colors and showed 28.04 (M1) and 25 per cent (MS2) antioxidant activities confirmed by DPPH reduction method.Pigments were evaluated for their potential as dye by dyeing cotton fabric material.The findings revealed less color loss when dye was used with mordant thiourea to dye cotton material.Thus, potential of biocolor from bacteria inhabiting hot water springs of Himachal Pradesh can be exploited in dyeing industries.

Source: Annals of Phytomedicine An International Journal

DOI: 10.21276/ap.2018.7.1.7

Authors: Meenakshi Meenakshi, Neerja Rana, Anjali Chauhan

Institution: Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Language: English

Topic: Microbial Metabolism and Applications

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W2884346830

FWCI: 0.154

Cites: 14

Cited by: 8

Related to: 10