Year: 2018
Type: article
Abstract: Point mutations are the cause of many human genetic diseases 1 . However, the typical indel mutations generated by CRISPR/Cas9 make this system not ideal for modeling point mutations. The recently developed base editors such as the third generation base editor (BE3) have proved effective for creating missense mutations and early stop codons 2 , 3 , and have been used in zebrafish, mice, and rabbits through zygote microinjection 4 , 5 , 6 , 7 . In this study, we demonstrated the feasibility of BE3 and somatic cell nuclear transfer (SCNT) in generating pig models.
Source: Cell Discovery
Authors: Zhifang Li, Xiaoyue Duan, Xiaomeng An, Tao Feng, Pan Li, Linlin Li, Jun Liu, Panxue Wu, Dengke Pan, Xuguang Du, Sen Wu
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,590
Awards: G5294828804, G8344082566
OpenAlex ID: W2900801532