Year: 2018
Type: conference-paper
Abstract: Alcohol is commonly found in food and beverage. This alcohol contents must be precisely measured to ensure the halalness of a product, considering the fatwa of Ulema's Council of Indonesia. Alcohol measurement can be carried out using alcohol biosensor based on microbe from pineapple juice, Saccharomyces cerevisiae yeast, which is known to produce alcohol dehydrogenase. This enzyme can reversibly convert ethanol to acetaldehyde. 1 % Ethanol measurement shows oxidation and reduction peak current at potential 0.736 V and 0.248 V, respectively. The result shows that immobilized electrode with OD600 value of 0.300 microbe cells generate the highest peak current. One of requirement sufficiently biosensor is high linearity. The best linearity measurement value is 0.9936 which is would be obtained at 0.01-2.50% concentration range. This linearity of ethanol measurement is similar with ethanol measurement using pure enzyme. The result would make this study potentially prospective.
Institutions: IPB University, Indonesian Institute of Sciences
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2901255274