Work

Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics

Year: 2018

Type: article

Abstract: Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.

Source: Chemistry - A European Journal

DOI: 10.1002/chem.201804488

Authors: Linda Cerofolini, Stefano Giuntini, Azzurra Carlon, Enrico Ravera, Vito Calderone, Marco Fragai, Giacomo Parigi, Claudio Luchinat

Institutions: Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine, University of Florence

Language: English

Topic: Enzyme Structure and Function

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: European Commission, Ministero dell’Istruzione, dell’Università e della Ricerca, Università degli Studi di Firenze, Fondazione Cassa di Risparmio di Firenze, Instituto de Física de Cantabria, Horizon 2020 Framework Programme

Awards: G1982653369, World-wide E-infrastructure for structural biology, Metodologie chimiche innovative per strategie molecolari avanzate in biomedicina, G6997527528, G735744980, A worldwide e-Infrastructure for NMR and structural biology, G823774000

OpenAlex ID: W2901650786

FWCI: 2.154

Cites: 51

Cited by: 48

Related to: 10