Biological characterization and molecular identification of virus belonging to the Tobamovirus genus isolated from Capsicum spp.
Year: 2003
Type: article
Abstract: Isolates belonging to the Tobamovirus genus collected from commercial sweet and hot peppers fields surrounding the cities of Lins, Sorocaba and Salto, Sao Paulo State, Brazil, were biologically characterized and identified by RT-PCR. The isolates were purified by single local lesion passages in Nicotiana glutinosa and maintained in Nicotiana cleevelandii plants through successive sap-inoculations. Using the differential genotypes of Capsicum spp., the isolates Pe-01, Pe-03, Pe-05 and Pe-12 could be classified as members of the P 1 - 2 pathotype. The isolate Pe-08 was shown to belong to the P 1 pathotype and Pe-02, Pe-04, Pe-06, Pe-07, Pe-09, Pe-10, Pe-11 and Pe-13, to the P 0 pathotype. Through RT-PCR using degenerated and specific primers to Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV) and Pepper mild mottle virus (PMMoV) it was possible to identify the specie of tobamovirus in almost isolates studied. Isolates Pe-06, Pe-07, Pe-09, Pe-10 and Pe-13 could be identified as ToMV and the isolates Pe-01, Pe-03, Pe-05 and Pe-12 as PMMoV. The TMV and ToMV primers indicated the presence of mixed infections in the isolate Pe-08. All isolates were positive for the universal primers, but Pe-02, Pe-04 and Pe-11 could not be detected by any one of the specific primers.
Source: Summa Phytopathologica
Language: English
Topic: Plant Virus Research Studies
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W290205286