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Autoimmunity and effector recognition in Arabidopsis thaliana can be uncoupled by mutations in the RRS1‐R immune receptor

Year: 2018
Type: article
Abstract: Summary Plant nucleotide‐binding leucine‐rich repeat (NLR) disease resistance proteins recognize specific pathogen effectors and activate a cellular defense program. In Arabidopsis thaliana (Arabidopsis), Resistance to Ralstonia solanacearum 1 (RRS1‐R) and Resistance to Pseudomonas syringae 4 (RPS4) function together to recognize the unrelated bacterial effectors PopP2 and AvrRps4. In the plant cell nucleus, the RRS1‐R/RPS4 complex binds to and signals the presence of AvrRps4 or PopP2. The exact mechanism underlying NLR signaling and immunity activation remains to be elucidated. Using genetic and biochemical approaches, we characterized the intragenic suppressors of sensitive to low humidity 1 (slh1), a temperature‐sensitive autoimmune allele of RRS1‐R. Our analyses identified five amino acid residues that contribute to RRS1‐RSLH1 autoactivity. We investigated the role of these residues in the RRS1‐R allele by genetic complementation, and found that C15 in the Toll/interleukin‐1 receptor (TIR) domain and L816 in the LRR domain were also important for effector recognition. Further characterization of the intragenic suppressive mutations located in the RRS1‐R TIR domain revealed differing requirements for RRS1‐R/RPS4‐dependent autoimmunity and effector‐triggered immunity. Our results provide novel information about the mechanisms which, in turn, hold an NLR protein complex inactive and allow adequate activation in the presence of pathogens.
DOI: 10.1111/nph.15617
Authors: Toby E. Newman, Jungmin Lee, Simon J. Williams, Sera Choi, Morgan K. Halane, Jun Zhou, Peter Solomon, Bostjan Kobe, Jonathan D. G. Jones, Cécile Segonzac, Kee Hoon Sohn
Institutions: Pohang University of Science and Technology, Massey University, Australian National University, The University of Queensland, Institute for Molecular Bioscience, Norwich Research Park, Sainsbury Laboratory, Seoul National University
Language: English
Subfield: Plant Science
Open Access status: bronze
Funders: National Research Foundation, Seoul National University, Rural Development Administration, National Research Foundation of Korea, Ministry of Science and ICT, South Korea, Medical Research Council, Biotechnology and Biological Sciences Research Council, Australian Research Council, National Health and Medical Research Council
Awards: Transcriptional regulation in effector-triggered immunity via NB-LRR resistance genes RPS4 & RRS1, G2024921812, G2207880632, G3675711857, Molecular mechanism of action of plant immune receptors, Structural biology and therapeutic targeting of proteins involved in infection and immunity, G4998647726, Domain/domain interactions in RPS4/RRS1 immune complex activation by bacterial effectors, How do effector proteins from necrotrophic fungi cause disease in plants?, Recognition
OpenAlex ID: W2903313402
FWCI: 1.075
Cites: 39
Cited by: 12
Related to: 10