Work

Application of Two-Dimensional Gel Electrophoresis in Combination with Mass Spectrometry in the Study of Hormone Proteoforms

Year: 2019

Type: book-chapter

Abstract: Hormone is a category of important endocrine regulatory proteins in human endocrine systems. Clarification of hormone proteoforms directly leads to understanding of its biological roles. Two-dimensional gel electrophoresis (2DGE) in combination with mass spectrometry (MS) plays important roles in identification of hormone proteoforms such as human growth hormone (hGH) proteoforms and human prolactin (hPRL) proteoforms. This book chapter will review the hormone proteoforms focusing on hGH and hPRL, the methodology of hormone proteoform study, and future perspective of human hormone proteoform study to find biomarkers for in-depth understanding of molecular mechanisms, and individualized and precise diagnosis, therapy, and prognostic assessment of hormone-related diseases.

Source: IntechOpen eBooks

DOI: 10.5772/intechopen.82524

Authors: Xianquan Zhan, Tian Zhou

Institutions: Central South University, Xiangya Hospital Central South University

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: National Natural Science Foundation of China, Natural Science Foundation of Hunan Province, Xiangya Hospital, Central South University