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Generation of Infectious Recombinant Human Rotaviruses from Just 11 Cloned cDNAs Encoding the Rotavirus Genome

Year: 2019
Type: article
Abstract: Human group A rotavirus (HuRVA) is a leading pathogen causing severe diarrhea in young children worldwide. In this paper, we describe the generation of recombinant HuRVA (strain KU) from only 11 cloned cDNAs encoding the HuRVA genome by reverse genetics. The growth properties of the recombinant HuRVA were similar to those of the parental RVA, providing a powerful tool for better understanding of HuRVA replication and pathogenesis. Furthermore, the ability to manipulate the genome of HuRVAs "to order" will be useful for next-generation vaccine production for this medically important virus and for the engineering of clinical vectors expressing any foreign genes.
DOI: 10.1128/jvi.02207-18
Authors: Satoshi Komoto, Saori Fukuda, Masanori Kugita, Riona Hatazawa, Chitose Koyama, Kazuhiko Katayama, Takayuki Murata, Koki Taniguchi
Language: English
Field: Medicine
Open Access status: green
Awards: Applications of entirely plasmid-based reverse genetics system on rotavirus research, G5443273433, G6097227810, G7448866251, Establishment and applications of an entirely plasmid-based reverse genetics system, G8102731819
OpenAlex ID: W2912199523
FWCI: 5.828
Cites: 40
Cited by: 50
Related to: 10