Additional file 5: Figure S5. of In vitro analyses of mitochondrial ATP/phosphate carriers from Arabidopsis thaliana revealed unexpected Ca2+-effects
Year: 2015
Type: article
Abstract: Impact of internal EGTA on ATP transport via AtAPC2. Uptake of 50 μM [α32P]-ATP into proteoliposomes loaded with Pi (black bars) or Pi plus 200 μM EGTA (light gray bars) was set to 100% (control). Inhibitory and stimulatory effects of externally added EGTA (50 μM) and CaCl2 (500 μM) on the corresponding transport rates were calculated accordingly. Reactivation of transport inhibited by external EGTA was induced by addition of 500 μM CaCl2. Transport (inhibition as well as activation) was allowed for 10 min. Data represent net values (ATP/Pi exchange minus background values of non-loaded proteoliposomes) and are the mean of at least three replicates. Standard errors are indicated. (PDF 252 kb)
Source: Figshare
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2916688917