Year: 2019
Type: article
Abstract: In forensic investigation, DNA plays a vital role to link the suspect/individual, victim and the scene of occurrence.DNA constitutes the genetic material of an individual and offers the preparation of DNA profile from the recovered biological materials often in sexual assault cases.This leads to apprehend the perpetrator of any crime based on the obtained DNA profile from such biological samples.It helps to identify the gender of an individual in missing identity and also in mixed samples.Structural polymorphism, which is rapidly being determined as the utmost form of genome variation is predominantly found on the Y chromosome.The gender from any sample is always determined by the DNA profile based on the presence of Y allele in amelogenin in autosomal STR in single marker of Y chromosome.The detection of any single Y chromosome in an unknown sample proves that, it belongs to a male individual.But in a few instances, it becomes a challenging task whenever due to mutation, no Y allele on amelogenin in male DNA profile in autosomal STR.In our previous study, we used the Identifiler plus Kit in the population of Delhi and observed that the profile of 708 samples was successfully obtained while only 2 profiles were observed without Y allele on amelogenin marker.The above two Y deleted samples were amplified by use of Globalfiler kit having 3 markers of Y chromosome.In such cases, Globalfiler kit helps to provide the presence of two other markers of Y chromosomes for determination of male gender of sample.
Authors: Naresh Kumar, Amit Chauhan, Ritika Gupta, SK Shukla
Institution: Amity University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2937698564