Copper nanoparticles stabilized with cashew gum: Antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell line
Year: 2019
Type: article
Abstract: Copper nanoparticles stabilized with cashew (CG-CuNPs) were synthesized by reduction reaction using ascorbic acid and sodium borohydride, using the cashew gum (CG) as a natural polymer stabilizer. Dynamic light scattering, atomic force microscopy, Fourier-transform infrared spectroscopy, UV-Vis spectrophotometry, and x-ray diffraction were used to characterize the nanoparticles (CG-CuNPs), and copper was quantified by electrochemical measurement. The UV-vis spectra of the CG-CuNPs confirmed the formation of nanoparticles by appearance of a surface plasmon band at 580 nm after 24 h of reaction. The Fourier-transform infrared spectrum of CG-CuNPs showed the peak at 1704 cm −1 from cashew gum, confirming the presence of the gum in the nanoparticles. The average size of CG-CuNPs by dynamic light scattering and atomic force microscopy was around 10 nm, indicating small, approximately spherical particles. Antimicrobial assays showed that CG-CuNPs had activity against Staphylococcus aureus ATCC 29213 with a minimal inhibitory concentration of 0.64 mM. The cytotoxicity assay on BALB/c murine macrophages showed lower cytotoxic effects for CG-CuNPs than CuSO 4 ·5H 2 O. Viability cell assays for CG-CuNPs at (0.250 mM) inhibited by 70% the growth of 4T1 LUC (4T1 mouse mammary tumor cell line) and NIH 3T3 cells (murine fibroblast cells) over a 24-h period. Therefore, CG-CuNPs can be used as an antimicrobial agent with lower cytotoxic effects than the CuSO 4 ·5H 2 O precursor.
Authors: Adriany Amorim, Ana Carolina Mafud, Silvania Nogueira, Joilson Ramos- Jesus, Alyne Rodrigues de Araújo, Alexandra Plácido, Maria Brito Neta, Michel Muálem Moraes Alves, Fernando Aécio Amorim Carvalho, Daniel Dias Rufino Arcanjo, Sacha Braun, Marta Sánchez-Paniagua López, Beatriz López-Ruiz, Cristina Delerue-Matos, Yvonne Mascarenhas, Durcilene Silva, Peter Eaton, José Roberto Souza Almeida Leite
Institutions: Universidade Federal do Piauí, Universidade de São Paulo, Instituto de Física de São Carlos, Universidade do Porto, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Universidade de Brasília, Universidad Complutense de Madrid, Rede de Química e Tecnologia, Universidade Técnica do Porto
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
Awards: G1582713205, G299573334, G3127338507, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G5905040377, RASTREABILIDADE DE ORGANISMOS GENETICAMENTE MODIFICADOS NA CADEIA ALIMENTAR: DESENVOLVIMENTO DE GENOSSENSORES COMO NOVAS ABORDAGENS PARA A SUA DETEÇÃO. alterado para “Traceability of genetically modified organisms in the food chain: Development of biosensing devices as new approaches for their detection”
OpenAlex ID: W2942952895