Work

Autologous hematopoietic stem cell transplantation in systemic sclerosis induces long-lasting changes in B cell homeostasis toward an anti-inflammatory B cell cytokine pattern

Year: 2019

Type: article

Abstract: Autologous hematopoietic stem cell transplantation (aHSCT) is performed in patients with aggressive forms of systemic sclerosis (SSc). The profile of B cell reconstitution after aHSCT is not fully understood. The aim of this study was to investigate changes of B cell subsets and cytokine production of B cells in patients with SSc after aHSCT. Peripheral blood of six patients with SSc was collected at defined intervals up to 16 months after aHSCT. Immunophenotyping was performed, and B cell function was determined by measuring cytokine secretion in supernatants of stimulated B cell cultures. Within 1 month after aHSCT, a peak in the percentage of CD38 ++ /CD10 + /IgD + transitional B cells and CD38 ++ /CD27 ++ /IgD − plasmablasts was detected. Long-term changes persisted up to 14 months after aHSCT and showed an increased percentage of total B cells; the absolute B cell number did not change significantly. Within the B cell compartment, an increased CD27/IgD + naïve B cell percentage was found whereas decreased percentages of CD27 + /IgD + pre-switched memory, CD27 + /IgD − post-switched memory, and CD27 − /IgD − double-negative B cells were seen after aHSCT. Cytokine secretion in B cell cultures showed significantly increased IL-10 concentrations 13 to 16 months after aHSCT. A changed composition of the B cell compartment is present for up to 14 months after aHSCT indicating positive persisting effects of aHSCT on B cell homeostasis. The cytokine secretion profile of B cells changes in the long term and shows an increased production of the immune regulatory cytokine IL-10 after aHSCT. These findings might promote the clinical improvements after aHSCT in SSc patients.

Source: Arthritis Research & Therapy

DOI: 10.1186/s13075-019-1889-8

Authors: Michael Gernert, Hans-Peter Tony, Eva Christina Schwaneck, Ottar Gadeholt, Marc Schmalzing

Institutions: University of Würzburg, Universitätsklinikum Würzburg

Language: English

Topic: Systemic Sclerosis and Related Diseases

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,288