Super-condenser enables labelfree nanoscopy
Year: 2019
Type: article
Abstract: in size via adiabatically tapered single-mode waveguides and tackle the overlap constraints of the Fourier ptychography phase retrieval algorithm two-fold: firstly, the directionality of the illumination wave vector is changed sequentially via a multiplexed input structure of the waveguide chip layout and secondly, the wave vector modulus is shortend via step-wise increases of the illumination light wavelength over the visible spectrum. We test the method in simulations and in experiments and provide details on the underlying image formation theory as well as the reconstruction algorithm. While the generated Fourier ptychography reconstructions are found to be prone to image artefacts, an alternative coherent imaging method, rotating coherent scattering microscopy (ROCS), is found to be more robust against artefacts but with less achievable resolution.
Source: Optics Express
DOI: 10.1364/oe.27.025280
Authors: Florian Ströhl, Ida S. Opstad, Jean-Claude Tinguely, Firehun T. Dullo, Ioanna Mela, Johannes W. M. Osterrieth, Balpreet S. Ahluwalia, Clemens F. Kaminski
Institutions: University of Cambridge, UiT The Arctic University of Norway
Language: Italian
Subfield: Radiation
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,270
Awards: G1484476120, G1835608419, MICA: Multi-parametric and super-resolution imaging of amyloidogenic proteins, Development of single molecule techniques for nanoscale imaging of toxic protein species in vitro and in cells., Liquid droplets and hydrogels: protein phase transition in health and disease, Mechanisms of neurotoxicity of amyloid aggregates., Next generation live super-resolution microscopy: development and application at the Cambridge Advanced Imaging Centre, Development of Deep-UV Quantitative Microscopy for the Study of Mitochondrial Dysfunction, G8039008064
OpenAlex ID: W2948754644