Characterization of Intact Proviruses in Blood and Lymph Node from HIV-Infected Individuals Undergoing Analytical Treatment Interruption
Year: 2019
Type: article
Abstract: HIV-1 persists as a latent infection in CD4 + T cells that can be found in lymphoid tissues in infected individuals during ART. However, the importance of this tissue reservoir and its contribution to viral rebound upon ART interruption are not clear. In this study, we sought to compare latent HIV-1 from blood and lymph node CD4 + T cells from five HIV-1-infected individuals. Further, we analyzed the contribution of lymph node viruses to viral rebound. We observed that the frequencies of intact proviruses were the same in blood and lymph node. Moreover, expanded clones of T cells bearing identical proviruses were found in blood and lymph node. These latent reservoir sequences did not appear to be the direct origin of rebound virus. Instead, latent proviruses were found to contribute to the rebound compartment by recombination.
Source: Journal of Virology
DOI: 10.1128/jvi.01920-18
Authors: Line K. Vibholm, Julio C.C. Lorenzi, Joy A. Pai, Yehuda Z. Cohen, Thiago Y. Oliveira, John P. Barton, Marco Garcia Noceda, Ching-Lan Lu, Yuria Ablanedo-Terrazas, Perla M. Del Rio Estrada, Gustavo Reyes Teran, Martin Tolstrup, Paul W. Denton, Tine Damsgaard, Ole S. Søgaard, Michel C. Nussenzweig
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Funders: Bill and Melinda Gates Foundation, City University of New York, Aarhus Universitet, Danmarks Frie Forskningsfond, National Institutes of Health, Center for AIDS Research, University of Washington, National Institute of Allergy and Infectious Diseases, Einstein-Rockefeller-CUNY Center for AIDS Research, Albert Einstein College of Medicine
Awards: BEAT-HIV: Delaney Collaboratory to Cure HIV-1 Infection by Combination Immunotherapy, G3190470567, G342479239, Exploring the dynamics and composition of the latent HIV reservoir by analyzing the diversity of the replication-competent reservoir, G629634775, Optimization of Fc effector activity of anti-HIV antibodies to target HIV reservoir, Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, G7353851086, G7381980221, ERC Einstein-Rockefeller-CUNY Center for AIDS research
OpenAlex ID: W2949650899