Metagenomic Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Central Nervous System Infections: A Multicentre Prospective Study
Year: 2019
Type: preprint
Study design: Observational Study
Abstract: ABSTRACT Background Infectious encephalitis and meningitis are often treated empirically without identification of the causative pathogen. Metagenomic next-generation sequencing (mNGS) is a high throughput technology that enables the detection of pathogens independent of prior clinical or laboratory information. Methods The present study was a multicentre prospective evaluation of mNGS of cerebrospinal fluid (CSF) for the diagnosis of suspected central nervous system infections. Results A total of 276 patients were enrolled in this study between Jan 1, 2017 and Jan 1, 2018. Identification of an etiologic pathogen in CSF by mNGS was achieved in 101 patients (36.6%). mNGS detected 11 bacterial species, 7 viral species, 2 fungal species, and 2 parasitic species. The five leading positive detections were varicella-zoster virus (17), Mycobacterium tuberculosis (14), herpes simplex virus 1 (12), Epstein-Barr virus (12), and Cryptococcus neoformans (7). False positives occurred in 12 (4.3%) patients with bacterial infections known to be widespread in hospital environments. False negatives occurred in 16 (5.8%) patients and included bacterial, viral and fungal aetiologies. Conclusions mNGS of CSF is a powerful diagnostic method to identify the pathogen for many central nervous system infections.
DOI: 10.1101/658047
Authors: Siyuan Fan, Xiaojuan Wang, Yafang Hu, Jingping Shi, Yueli Zou, Weili Zhao, Xiaodong Qiao, Chunjuan Wang, Jerome H. Chin, Lei Liu, Lingzhi Qin, Shengnan Wang, Hongfang Li, Wei Yue, Weihe Zhang, Xiaohua Li, Ying Ge, Honglong Wu, Weijun Chen, Yongjun Li, Tianjia Guan, Shiying Li, Yihan Wu, Gaoya Zhou, Zheng Liu, Yushun Piao, Jianzhao Zhang, Changhong Ren, Li Cui, Caiyun Liu, Haitao Ren, Yanhuan Zhao, Shuo Feng, Haishan Jiang, Jiawei Wang, Hui Bu, Shougang Guo, Bin Peng, Liying Cui, Wei Li, Hongzhi Guan
Institutions: Chinese Academy of Medical Sciences & Peking Union Medical College, Peking Union Medical College Hospital, Zhengzhou University, Henan Provincial People's Hospital, Nanfang Hospital, Southern Medical University, Nanjing Medical University, Second Hospital of Hebei Medical University, Chifeng University, Shandong University, Shandong Provincial Hospital, NYU Langone Health, New York University, Beijing Tongren Hospital, Capital Medical University, Affiliated Hospital of Jining Medical University, Tianjin Huanhu Hospital, China-Japan Friendship Hospital, Inner Mongolia Medical University, BGI Group (China), Chinese Academy of Sciences, Beijing Institute of Genomics, University of Chinese Academy of Sciences, BGI Genomics, North China University of Science and Technology, Inner Mongolia People's Hospital, Hunan Institute of Mental Health, Xuan Wu Hospital of the Capital Medical University, Capital Institute of Pediatrics, Children's Hospital of Capital Institute of Pediatrics, Beijing Children’s Hospital, First Hospital of Jilin University
Language: English
Subfield: Clinical Biochemistry
Domain: Life Sciences
Open Access status: green
Awards: G2663675820, G6178506094
OpenAlex ID: W2952012306