B cell immunophenotyping reveals decreased frequency of CD11c+ B cell expressing FcRL5+ subset in naturally malaria-exposed Fulani adults.
Year: 2019
Type: other
Abstract: In malaria endemic area antibody response is the hallmark of repeated exposition to the parasite. People of the Fulani ethnic group are more resistant to malaria compared with genetically distinct ethnic groups, such as the Mossi people, in West Africa. We have previously demonstrated that the Fulani mount an enhanced and broadly reactive immunoglobulin (Ig)M. In order to investigate which B cell subsets are contributing to this enhanced IgM response, we characterized circulating B cell populations in naturally malaria-exposed Fulani and Mossi adults of Burkina Faso. During the low malaria transmission season from 15/03/2019 to 05/04/2019, we carried out a cross sectional study in 30 Fulani and 30 Mossi adults from the village of Barkoundouba. Peripheral Blood Mononuclear cells (PBMCs) were collected, stained using anti-CD21 FITC, anti-FcRL5 PE, anti-CD11c PE CY7, anti-T-Bet PE Txr, anti CD27 BV421, anti CD19 PerCP CY5.5, anti CD10 APC, anti-CD20 APC-H7 and IgM BV510. The immunophenotyping of B cells was performed using the BD LSR II Flow cytometer. We found that naïve B-cell IgM+ (“40.96 ± 1.72 N=29” vs “53.84 ± 1.84 N=30”; p=0.0001), the atypical Memory B-Cell (MBC) IgM+(“15.73 ± 1.81 N=29” vs “34.64 ± 2.57 N=30”; p=0.00002) and the CD11c+ FCRL5+IgM+ subsets (“6.73 ± 1.23 N=29” vs “19.22 ± 2.40 N=30”; p=0.000013) frequencies were significantly higher in the Fulani than in Mossi. However, the CD11c+ B cell subset expressing FcRL5 (“34.08 ± 1.70 N=29” vs “23.96 ± 1.15 N=30”; p=0.0001) frequencies was significantly lower in Fulani than in Mossi. These results identify CD11c+ B cell expressing FcRL5+ subsetas a marker that may further highlight differences in Fulani versus Mossi adults.
Language: English
Topic: Malaria Research and Control
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2955782857