Year: 2019
Type: article
Abstract: Molecular mechanisms governing cell fate decision events in bone marrow mesenchymal stromal cells (MSC) are still poorly understood. Herein, we investigated the homeobox gene Prep1 as a candidate regulatory molecule, by adopting Prep1 hypomorphic mice as a model to investigate the effects of Prep1 downregulation, using in vitro and in vivo assays, including the innovative single cell RNA sequencing technology. Taken together, our findings indicate that low levels of Prep1 are associated to enhanced adipogenesis and a concomitant reduced osteogenesis in the bone marrow, suggesting Prep1 as a potential regulator of the adipo-osteogenic differentiation of mesenchymal stromal cells. Furthermore, our data suggest that in vivo decreased Prep1 gene dosage favors a pro-adipogenic phenotype and induces a “browning” effect in all fat tissues.
DOI: 10.3390/ijms20153639
Institutions: National University of Singapore, Institute of Biomedical Technologies, National Research Council, Istituto di Fisiologia Clinica, Harvard University, University of Pisa, IFOM, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Beth Israel Deaconess Medical Center, Harvard Stem Cell Institute
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,824
Award: G6178294806
OpenAlex ID: W2962935485