Work

Therapeutic Effect of Curcumin, a Plant Polyphenol Extracted From Curcuma longae , in Fibroblasts From Patients With Graves' Orbitopathy

Year: 2019

Type: article

Abstract: Purpose: We examined the therapeutic effect of nontoxic concentrations of curcumin, a plant polyphenol extracted from Curcuma longae, in primary cultures of orbital fibroblasts from Graves' orbitopathy (GO). Methods: The effect of curcumin on interleukin (IL)-1β induced-proinflammatory cytokine production was determined using quantitative real-time PCR, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis. Adipogenic differentiation was identified using Oil-Red O staining, and levels of peroxisome proliferator activator γ (PPARγ) and CCAAT-enhancer-binding proteins (C/EBP) α/β were determined by Western blot analyses. Antioxidant activity was measured using an oxidant-sensitive fluorescent probe to detect intracellular reactive oxygen species (ROS) generated in response to hydrogen peroxide (H2O2) and cigarette smoke extract (CSE). Results: Treatment with curcumin resulted in a dose- and time-dependent decrease in IL-1β-induced synthesis of inflammatory cytokines, including IL-6, IL-8, MCP-1, and ICAM-1 at both mRNA and protein levels. Decrease in lipid droplets and expression of PPARγ and c/EBPα/β were noted in fibroblasts treated with curcumin during adipose differentiation. CSE- or H2O2-induced ROS synthesis was significantly lower in curcumin-treated fibroblasts in comparison with the control. Curcumin significantly suppressed IL-1β-induced phosphorylated extracellular signal-regulated kinase, Akt, c-Jun NH(2)-terminal kinase, and nuclear factor κ-light-chain-enhancer of activated B cells, p65 proteins and stimulated β-catenin translocation into nucleus during adipogenesis. Conclusions: Curcumin inhibits proinflammatory cytokine production, ROS synthesis, and adipogenesis in orbital fibroblasts of GO patients in vitro possibly related to multiple proinflammatory signaling molecules and β-catenin pathway. The results of the study support potential use of the curcumin in the treatment of GO.

Source: Investigative Ophthalmology & Visual Science

DOI: 10.1167/iovs.19-27376

Authors: Jihei Sara Lee, Jinjoo Kim, Eun Jig Lee, Jin Sook Yoon

Institutions: Yonsei University, Severance Hospital

Language: English

Topic: Ophthalmology and Eye Disorders

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,850