Design strategy for germanium-rhodamine based pH-activatable near-infrared fluorescence probes suitable for biological applications
Year: 2019
Type: article
Abstract: Fluorescence probes that can detect pH are useful tools for biological research and clinical diagnosis. Here we report pH-activatable near-infrared fluorescence probes, based on hydroxymethyl germanium-rhodamine (HMGeR), that are suitable for a range of biological applications. The p K a , the ratio of the fluorescent form in an acidic environment, and the absorption/emission wavelengths can all be conveniently optimized. The most promising probe, 2-HM IGeR, offers significant advantages over currently available near-infrared pH probes, notably high quantum efficiency, appropriate p K a value for biological applications, and high photostability. Further, our molecular design strategy allows easy conjugation of the probes to biomolecules without loss of functionality. We illustrate the value of this strategy by developing probe-Herceptin® and probe-avidin conjugates to visualize pH change in cellular vesicles during endocytosis, and to visualize tumors in a mouse model, respectively. We believe 2-HM IGeR is currently among the best-in-class pH-activatable near-infrared probes for biological and medical research.
Source: Communications Chemistry
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,170
Awards: Development of synthetic photo-functional molecules for medical applications, G1980492409, G2532640398, Development of novel luciferin analogues for utilizing luciferases as new functional tools in vivo, G2997796445, G3050258952, G3621080155, Design, synthesis and application of fluorescent probes for the studies of drug discovery and medicine, G5866139558, Comprehensive search of cancer specific enzymatic activities and creation of innovative neutron capture therapy probe, G8779124241, G8899196992
OpenAlex ID: W2968960280