Inferred family structure of an endangered species, Pinna nobilis, using molecular analyses: implications of connectivity for conservation
Year: 2019
Type: article
Abstract: Event Abstract Back to Event Inferred family structure of an endangered species, Pinna nobilis, using molecular analyses: implications of connectivity for conservation Elisabet Nebot1, 2*, Maite Vázquez1*, Emilie Boissin2, Claire Peyran2, Salud Deudero1 and Serge Planes2 1 Centro Oceanográfico de Baleares, Instituto Español de Oceanografía, Spain 2 Centre de Recherches Insulaires et Observatoire de l'Environnement CRIOBE - USR 3278, French Polynesia PROBLEM STATEMENT Coastal Mediterranean marine biodiversity is under threat due to several anthropogenic impacts. Marine protected areas (MPAs) are an essential tool to counteract these impacts by preserving biodiversity, natural habitats and maintaining genetic diversity. Knowledge of the genetic structure and connectivity patterns of keystone and vulnerable species is essential, since it helps on the preservation of the structure and function of ecosystems (Daily et al., 2000; Libralato et al., 2010). Here we focus on the genetic structure and connectivity patterns of the emblematic and endemic bivalve Pinna nobilis (Linnaeus, 1758) in a MPA from the Western Mediterranean. Recently, in autumn of 2016, the occurrence of a new parasite species (Haplosporidium pinnae, Catanese et al., 2018), is producing a mass mortality event (MME) in the whole Mediterranean Sea. As a result, Pinna nobilis has been declared as critically endangered species in Spain. Given its current conservation status, and the low knowledge of their genetic structure and connectivity at a local level, there is an urgent need to know more about these topics, in order to be able to proposed proper conservation measures for its recovery. METHODOLOGY The study was carried out in the Marine National Park of Cabrera, situated in the south of Mallorca. A total of 771 Pinna nobilis individuals were sampled from nine different sites between 2011 and 2014 (Vázquez-Luis et al., 2014), representing a low percentage of the population inhabiting the MPA before the MME (Vázquez-Luis et al., 2017). Total genomic DNA was extracted for each individual and genotyped at 23 polymorphic microsatellite loci. Population genetic analysis and parentage analysis were performed. RESULTS Pinna nobilis individuals from the National Park of Cabrera were grouped into one single panmictic population. Of the 771 sampled individuals, almost half (45.01%) of them were related with other individuals. Estells de Dins (EDD) had the highest number of related individuals (63.16%), while Dimoni (DIM) showed the lowest number of related individuals (36.84%) (Fig. 1). In total, 562 relationships were detected, mostly occurring in Santa Maria (STM, 60.14%) and Puerto (PORT, 14.95%). Specifically, most of the relationships in Santa Maria were between individuals from Santa Maria (57.99%), Santa Maria - Puerto (16.57%) and Santa Maria - Olla (7.69%). While most of the relationships in Puerto were between individuals from Santa-Maria (66.67%) and between individuals from Puerto itself (9.52%). In total 126 families were found, composed from 2 to 8 individuals, 66.67% of the families were composed with 2 individuals, 12.70% were composed with 3 individuals and only 1 family was composed with 8 individuals (Fig. 2). The maximum number of different sampled sites per family were four. In addition, no parents-offspring relationships were detected. MAJOR CONCLUSIONS A high number of individuals related have been found despite the low percentage of sampled individuals, concurring with the lack of genetic structure at the scale of Cabrera and pointing to high levels of connectivity of all sampled sites in the National Park. The high percentage of families with 2 members could be due to the reproductive characteristics of the species (external fecundation), to the difficulty of sampling a high percentage of the population (due to the high densities existing previous to the MME), the existence of pelagic larvae (dispersal process); and to the high mortality rates of the first years. However, the existence of families with a total of 8 members living in up to four different sites points out the high connectivity across the National Park. Moreover, based on results obtained, Santa Maria seems to be a source site with high number of connections to the rest of the sites within the National Park, making it a key site for future restocking programmes. FIGURE LEGENDS Figure. 1. Study area and percentage of related individuals per site Figure. 2 Number of individuals per family Figure 1 Figure 2 Acknowledgements This research was partially funded by research Project “Estado de conservación del bivalvo amenazado Pinna nobilis en el Parque Nacional del Archipiélago de Cabrera” (024/2010), “OAPM, MARM”. Thanks are due to the staff of Cabrera NP for giving permission and facilities. MVL was supported by a postdoctoral contract co-funded by the Regional Government of Balearic Islands and European Social Fund 2014–2020 and a postdoctoral contract Juan de la Cierva-Incorporación (IJCI-2016-29329) of MICINN. References Catanese, G., Grau, A., Valencia, J. M., Garcia-March, J. R., Vázquez-Luis, M., Alvarez, E., et al. 2018. Haplosporidium pinnae sp. nov., a haplosporidan parasite associated with mass mortalities of the fan mussel, Pinna nobilis, in the Western Mediterranean Sea. Journal of invertebrate pathology, 157, 9-24. doi: 10.1016/j.jip.2018.07.006 Daily, G.C., Soderqvist, T., Aniyar, S., Arrow, K., Dasgupta, P., Ehrlich, P.R., et al. 2000. The value of nature and the nature value. Science, 289, 395-396. doi: 10.1126/science.289.5478.395 Libralato, S., Coll, M., Tempesta, M., Santojanni, A., Spoto, M., Palomera., et al. 2010. Food-web traits of protected and exploited areas of the Adriatic Sea. Biological Conservation 143, 2182-2194. doi: 10.1016/j.biocon.2010.06.002 Vázquez-Luis, M., March, D., Álvarez, E., Álvarez-Berastegui, D., Deudero, S. 2014. Modelling spatial distribution of the endangered bivalve Pinna nobilis in a Marine Protected Area. Mediterranean Marine Science, 15/3, 626-634. doi: 10.12681/mms.796 Vázquez-Luis, M., Álvarez, E., Barrajón, A., García-March, J. R., Grau, A., Hendriks, I. E., et al. 2017. SOS Pinna nobilis: a mass mortality event in western Mediterranean Sea. Frontiers in Marine Science, 4, 220. doi: 10.3389/fmars.2017.00220 Keywords: Pinnidae, Bivalve, parentage analysis, Kinship analysis, Endemic species Conference: XX Iberian Symposium on Marine Biology Studies (SIEBM XX) , Braga, Portugal, 9 Sep - 12 Sep, 2019. Presentation Type: Oral Presentation Topic: Ecology, Biodiversity and Vulnerable Ecosystems Citation: Nebot E, Vázquez M, Boissin E, Peyran C, Deudero S and Planes S (2019). Inferred family structure of an endangered species, Pinna nobilis, using molecular analyses: implications of connectivity for conservation. Front. Mar. Sci. Conference Abstract: XX Iberian Symposium on Marine Biology Studies (SIEBM XX) . doi: 10.3389/conf.fmars.2019.08.00025 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2019; Published Online: 27 Sep 2019. * Correspondence: Mrs. Elisabet Nebot, Centro Oceanográfico de Baleares, Instituto Español de Oceanografía, Palma, Spain, [email protected] Dr. Maite Vázquez, Centro Oceanográfico de Baleares, Instituto Español de Oceanografía, Palma, Spain, [email protected] Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Elisabet Nebot Maite Vázquez Emilie Boissin Claire Peyran Salud Deudero Serge Planes Google Elisabet Nebot Maite Vázquez Emilie Boissin Claire Peyran Salud Deudero Serge Planes Google Scholar Elisabet Nebot Maite Vázquez Emilie Boissin Claire Peyran Salud Deudero Serge Planes PubMed Elisabet Nebot Maite Vázquez Emilie Boissin Claire Peyran Salud Deudero Serge Planes Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Source: Frontiers in Marine Science
Authors: Elisabet Nebot, Maite Vázquez, Emilie Boissin, Claire Peyran, Salud Deudero, Serge Planes
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,490
OpenAlex ID: W2976100855