Rapid Transduction and Expansion of Transduced T Cells with Maintenance of Central Memory Populations
Year: 2019
Type: article
Abstract: Chimeric antigen receptor (CAR)-T cells show great promise in treating cancers and viral infections. However, most protocols developed to expand T cells require relatively long periods of time in culture, potentially leading to progression toward populations of terminally differentiated effector memory cells. Here, we describe in detail a 9-day protocol for CAR gene transduction and expansion of primary rhesus macaque peripheral blood mononuclear cells (PBMCs). Cells produced and expanded with this method show high levels of viability, high levels of co-expression of two transduced genes, retention of the central memory phenotype, and sufficient quantity for immunotherapeutic infusion of 1–2 × 10 8 cells/kg in a 10 kg rhesus macaque. This 9-day protocol may be broadly used for CAR-T cell and other T cell immunotherapy approaches to decrease culture time and increase maintenance of central memory populations.
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,500
Awards: BELIEVE: Bench to Bed Enhanced Lymphocyte Infusions to Engineer Viral Eradication, Targeting virus-specific CAR T cells to lymphoid follicles to achieve durable HIV suppression, MIN-REACH Research Evaluation and Commercialization Hub, G6376637721, Mechanisms Underlying Persistent Lentivirus Replication in Follicular T Cells, Resource fo Nonhuman Primate Cell Depleting Antibodies, Wisconsin National Primate Research Center Support, Nonhuman Primate Reagent Resource, Wisconsin National Primate Research Center Support
OpenAlex ID: W2978763546