Work

Extended-Spectrumβ-Lactamases among Enterobacteriaceae Isolated from Urinary Tract Infections in Gaza Strip, Palestine

Year: 2019

Type: article

Abstract: Background. Extended-spectrumβ-lactamase-producing organisms causing urinary tract infections are increasing in incidence and pose a major impendence to health-care facility, having limited therapeutic options. This study aimed to assess the prevalence of ESBLs in Enterobacteriaceae isolates causing urinary tract infections in Gaza strip, Palestine, and to characterizeβ-lactamase types and associated resistance genes.Methods. Eighty-five Enterobacteriaceae isolates were recovered from urinary tract infections within three months in Gaza Strip hospitals. The characterization ofβ-lactamase genes and the genetic environments of CTX-M, the identification of associated resistance genes, and the presence and characterization of integrons were tested by PCR and sequencing.Results. The occurrence rate of ESBL among tested isolates was 30 (35.3%), and among ESBL-positive isolates,blaCTX-Mwas the highest followed byblaTEM. ESBL-CTX-M-1 group was confirmed in 93.3%, and the remaining carried CTX-M-9 group. CTX-M-15, CTX-M-3, CTX-M-1, CTX-M-14, CTX-M-27, and CTX-M-37 enzymes were demonstrated among the isolates with the majority (73%) being CTX-M-15. ISEcp-1was demonstrated in 27 (90%, high incidence) of ESBL isolates. Class 1 integrons have been detected in higher rates (53.3%) in ESBL-positive isolates in comparison with non-ESBL isolates (6, 33.3%). Cassettes of integron-1 contain (aadA1,aadA2,aadA5,dfrA5,dfrA7,dfrA12, anddfrA17) genes. Theaac(6′)-Ib-crgene was demonstrated in 36.7% of ESBL-positive isolates.Conclusions. This study indicates thatblaCTX-M-15was the most prevalentβ-lactamase in this region. Our study demonstrates for the first time in Palestine the identification ofblaCTX-M-15inP. rettgeriandS. liquefaciens, alsoblaCTX-M-37inE. cloacae.The coexpression of multipleβ-lactamase genes withaac(6′)-Ib-crandqnrin the presence of ISEcp-1and integrons in individual strains will increase the dissemination of highly resistant strains. ESBL producers were more resistant than non-ESBLs producers for almost all tested antibiotics.

Source: BioMed Research International

DOI: 10.1155/2019/4041801

Authors: Ghassan Tayh, Nahed Al Laham, Houssem Ben Yahia, Rym Ben Sallem, Abed Elkader Elottol, Karim Ben Slama

Institutions: Tunis El Manar University, Al-Azhar University – Gaza

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Microbiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,680