RAPD Analysis of Colletotrichum capsici Causing Anthracnose of Chilli in Madhya Pradesh, India
Year: 2019
Type: article
Abstract: In the present investigation an attempt was carry out to test the pathogenicity of the sixteen isolates of C. capsici obtained from different districts of Madhya Pradesh on ripe and green chilli by pin prick method and the genetically variation of these isolates was also detected by Random Amplified Polymorphic DNA (RAPD) technique. On ripe chilli, significantly maximum anthracnose incidence was recorded in BHP-1 (56.60 %) and minimum anthracnose incidence was recorded in CHN-2 (33.80 %) whereas on green chilli, significantly maximum anthracnose incidence was recorded in JHB-2 (89.60 %) while minimum anthracnose incidence was recorded in CHN-2 (61.40 %). For genetic variability, two primers were used which produced 32 scorable bands in which 23 bands were polymorphic and showed 72 % polymorphism. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into two clusters (I and II), confirming the genetic diversity among the isolates of C. capsici. The genetical similarity coefficient value ranged from 0.335 – 0.992 for OPD 07 primer across 7 isolates of C. capsici. RAPD analysis showed a clear difference in different isolates of C. capsici.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2984002002