Work

Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells.

Year: 2020

Type: article

Abstract: OBJECTIVE: Mesenchymal stem cells (MSCs) have prominent immunomodulatory roles in the tumor microenvironment. The current study intended to elucidate Treg subsets and their cytokines after exposing naïve T lymphocytes to adiposederived MSCs (ASCs). MATERIALS AND METHODS: T cells were then co-cultured with ASCs for five days. The phenotype of regulatory T cells (Tregs) and production of interleukine-10 (IL-10), transforming growth factor beta (TGF-β) and IL-17 were assessed using flow cytometry and ELISPOT assays, respectively. RESULT: Tregs were expanded after co-culturing of T cells with both cancer-ASCs and normal-ASCs, while they were statistically significant only in the presence of cancer-ASCs (P<0.05). Production of IL-10, IL-17 and TGF-β by T cells was increased in the presence of either normal- or cancer-ASCs; however, significant effect was only observed in the IL-10 and TGF-β of cancer-ASCs (P<0.05). CONCLUSION: The results further confirm the immunosuppressive impacts of ASCs on T lymphocytes and direct them to specific regulatory phenotypes which may support immune evasion and tumor growth.

Source: PubMed Central

DOI: 10.22074/cellj.2020.6313

Authors: Maryam Fakhimi, Abdol Rasoul Talei, Abbas Ghaderi, Mojtaba Habibagahi, Mahboobeh Razmkhah

Institutions: Shiraz University, Shiraz University of Medical Sciences

Language: English

Topic: Mesenchymal stem cell research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Shiraz University of Medical Sciences, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences