Year: 2019
Type: article
Abstract: Although the high-fat-diet-induced metabolic syndrome (MetS) is a precursor of human cardiac pathology, the myocardial metabolic state in MetS is far from clear. The discrepancies in metabolite handling between human and small animal models and the difficulties inherent in obtaining human tissue complicate the identification of the myocardium-specific metabolic response in patients. Here we use the large animal model of swine that develops the hallmark criteria of human MetS. Our comparative metabolomics together with transcriptomics and computational nonnegative matrix factorization (NMF) interpretation of the data exposes significant decline in metabolites related to the fatty acid oxidation, glycolysis, and pentose phosphate pathway. Behind the reversal lies decreased expression of enzymes that operate in the pathways. We showed that diminished glycogen deposition is a metabolic signature of MetS in the pig myocardium. The depletion of glycogen arises from disbalance in expression of genes that break down and synthesize glycogen. We show robust acetoacetate accumulation and activated expression of key enzymes in ketone body formation, catabolism and transporters, suggesting a shift in fuel utilization in MetS. A contrasting enrichment in O-GlcNAcylated proteins uncovers hexosamine pathway and O-GlcNAcase (OGA) expression involvement in the myocardial response to MetS. Although the hexosamine biosynthetic pathway (HBP) activity and the availability of the UDP-GlcNAc substrate in the MetS myocardium is low, the level of O-GlcNacylated proteins is high as the O-GlcNacase is significantly diminished. Our data support the perception of transcriptionally driven myocardial alterations in expression of standard fatty acids, glucose metabolism, glycogen, and ketone body related enzymes and subsequent paucity of their metabolite products in MetS. This aberrant energy metabolism in the MetS myocardium provide insight into the pathogenesis of CVD in MetS.
Source: PLoS ONE
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,595
Funders: Foundation for the National Institutes of Health, U.S. Department of Energy, National Institutes of Health, National Nuclear Security Administration, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, Laboratory Directed Research and Development, Los Alamos National Laboratory
Awards: G1430761085, G2234260717, G2414246456, G286898171, G2980544840, G310521049, G3336154855, G3636425355, G3700687868, G4169061630, G4844714250, G5657628048, G5713387433, SKca/IKca Channel Activation and Endothelial Protection During Cardiac Surgery, G6757401087, Metabolic Regulation of SKCa/IKCa Channels and Endothelial Function, G75892134, Effect of Cardioplegia and Cardiopulmonary Bypass on Coronary Microvascular Reactivity, Molecular Pathogenesis of the Hamartoma Syndromes, Vascular Dysfunction in Myocardial Ischemia and Metabolic Syndrome, Indiana Diabetes Research Center
OpenAlex ID: W2991253552