Chemical composition and antibacterial activity of honey collected from East Nusa Tenggara, Indonesia on pathogenic bacteria in aquaculture
Year: 2019
Type: conference-paper
Abstract: The objectives of this research were to investigate the chemical composition and the antibacterial activity of honey from East Nusa Tenggara, produced by Trigona spp., on pathogenic bacteria in fish. The honey used in this research was collected specifically from Semau and Timor Islands while Aeromonas hydrophila and Vibrio alginoliticus were used as the testing bacteria. The chemical composition tests included moisture content (gravimetric), pH, total sugar (spectrophotometric). The honey was tested for the presence of the following active compounds: alkaloids, flavonoids, tanin, saponins, steroids and terpenoids. Antibacterial activity tests were conducted using “paper disk” method with undiluted honey samples. The moisture content of the Semau honey (33 %) was higher than that of the Timor honey (24.65 %), but the total sugar of the Semau honey ( 6.62 %) was lower than that of the Timor honey (74.22%). All honey from both sources (Semau and Timor) in East Nusa Tenggara contained alkaloids, steroids and terpenoids but did not contain flavonoids, tannins and saponins. Antibacterial tests showed that the honey from both sources, Semau and Timor, showed antibacterial activity against V. Alginoliticus and A. hydrophila; the V. alginolitycus inhibition zone diameters were 8.5 mm and 10.5 mm, respectively, while and the A. hydrophila inhibition zone diameter was 8 mm for both honeys.
Author: Y Salosso
Institution: University of Nusa Cendana
Language: English
Subfield: Insect Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2993487906