The effect of plant growth regulators and different types of explants on in vitro regeneration of sweet pepper (Capsicum annuum L.)
Year: 2014
Type: article
Abstract: Present study was conducted to standardize a protocol for in vitro plant regeneration in Capsicum annuum L. cv California Wonder. Three different types of explants (Hypocotyl, Cotyledon and Cotyledonary petiole) from 8day-old seedling were used. Various concentrations of BA (2, 3 mgL-1), IBA (0.1, 0.2 mgL-1) and NAA (0.1, 0.2 mgL -1 ) were used for shoot regeneration. The highest number of shoot per explant was obtained from cotyledonary petiole on a MS medium supplemented with 3 mgL-1 BA and 0.2 mgL-1 IBA. Effective in vitro shoot elongation was achieved on half strength MS medium containing 0.1 mgL-1 BA in combination with 0.5 mgL-1 GA3. As well as adding activated charcoal (3 gL -1 ) and citric acid along with ascorbic acid caused eliminate phenolic material and prevent shoot necrosis in sweet pepper. Elongated shoots were rooted on half strength MS medium supplemented with 1 mgL-1 IBA.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W2993559985