Work

Development of Reverse Transcription Semi-nested PCR Primer Pairs for the Specific and Highly Sensitive Detection of Human Aichivirus A1

Year: 2019

Type: article

Abstract: Human Aichivirus A1 (HuAiV-A1) is a waterborne human pathogenic virus classified as Picornaviridae and Kobuvirus.In this study, we developed a method that can detect about 35 minutes faster with the same detection sensitivity level than the previously reported HuAiV-A1 diagnostic RT-PCR primer.The RT-PCR primer sets developed in this study are capable of detecting HuAiV-A1 at a level of about 100 ag and formed 563 bp amplification product.In addition, the RT-nested PCR method was able to amplify 410 bp using the RT-PCR product as a template.The detection sensitivity of our method was 10 times higher than the method with the highest detection sensitivity to date.Therefore, the detection method of HuAiV-A1 developed in this study is expected to be used in the water environment in which a small amount of virus exists.Also, this detection method is expected to be used as HuAiV-A1 diagnostic technology in both clinical and non-clinical field.

Source: Journal of Experimental & Biomedical Sciences/Biomedical Science Letters

DOI: 10.15616/bsl.2019.25.4.331

Authors: Siwon Lee, Kyu Bong Cho

Institution: Shinhan University

Language: English

Topic: Viral gastroenteritis research and epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Ministry of SMEs and Startups