Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells
Year: 2020
Type: article
Abstract: The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.
Source: Science Advances
Authors: Stephen D. Carter, Cheri M. Hampton, Robert Langlois, Roberto Melero, Zachary J. Farino, Michael J. Calderon, Wen Li, Callen T. Wallace, Ngoc Han Tran, Robert A. Grassucci, Stephanie E. Siegmund, Joshua Pemberton, Travis J. Morgenstern, Leanna Eisenman, Jenny I. Aguilar, Nili L. Greenberg, Elana S. Levy, Edward Yi, William G. Mitchell, William J. Rice, Christoph Wigge, Jyotsna Pilli, Emily W. George, Despoina Aslanoglou, Maïté Courel, Robin J. Freyberg, Jonathan A. Javitch, Zachary P. Wills, Estela Area-Gomez, Sruti Shiva, Francesca Bartolini, Allen Volchuk, Sandra A. Murray, Meir Aridor, Kenneth N. Fish, Peter Walter, Tamas Balla, Deborah Fass, Sharon G. Wolf, Simon C. Watkins, José María Carazo, Grant J. Jensen, Joachim Frank, Zachary Freyberg
Institutions: California Institute of Technology, Columbia University, Consejo Superior de Investigaciones Científicas, Centro Nacional de Biotecnología, University of Pittsburgh, Howard Hughes Medical Institute, University of California, San Francisco, Columbia University Irving Medical Center, National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development, New York State Psychiatric Institute, New York Structural Biology Center, Centre National de la Recherche Scientifique, Laboratoire de Biologie du Développement, Institut de Biologie Paris-Seine, Université Pierre-et-Marie-Curie, Hospital for Sick Children, Weizmann Institute of Science
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,500
Funders: National Science Foundation, U.S. Department of Defense, Howard Hughes Medical Institute, Pittsburgh Foundation, California Institute of Technology, Leon Levy Foundation, Comunidad de Madrid, Innovative Research Group Project of the National Natural Science Foundation of China, European Commission, Weizmann Institute of Science, Ministerio de Economía y Competitividad, Israel Science Foundation, National Institutes of Health, Agencia Estatal de Investigación, National Institute on Aging, National Institute on Drug Abuse, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, National Center for Research Resources, FP7 Ideas: European Research Council
Awards: National Resource for Automated Molecular Microscopy, Endoplasmic Reticulum Mitochondrial membranes in Alzheimer's disease, Request for Ultracryomicrotomy equipment, G1276305609, Imaging large macromolecular complexes inside cells with electron cryotomography, G1416151005, G1493310965, G1555047976, The Role of NgR Mediated Inhibition of New Synapse Assembly in Amyloid Beta Peptide Dependent Learning Deficits, Role of C99 in the regulation of lipid metabolism- Relevance to Alzheimer's disease, JEOL JEM-1011 Transmission Electron Microscope, G2908651626, G2999167203, Structural Analysis of Macromolecular Assemblies, G3062763459, G3267562521, Frontiers of Oxidative Protein Folding and Assembly: Catalysis of Disulfide Formation Downstream of the Endoplasmic Reticulum, Center for the Structural Biology of Cellular Host Elements in Egress, Trafficking, and Assembly of HIV (CHEETAH Center), A Synchroton Radiation Structural Biology Resource, G4418009050, Dual-Beam Scanning Electron Microscope for New York Structural Biology Center, Revealing Novel Mechanisms of Amphetamine Action in a Drosophila Model, Pharmacologic Treatment of Human Mitochondrial DNA (mtDNA) Disease, Investigation of the Regulation of Annular Gap Junction Processing, a Critical Modulator of Cell-Cell Communication, STRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLY, G533446661, Center for the Structural Biology of Cellular Host Elements in Egress, Trafficking, and Assembly of HIV (CHEETAH Center), G5796282054, G5804439651, G5967282749, G6011641542, G6898049487, G7077397202, Request for transmission electron microscope for the University of Pittsburgh, G79964497, G9000014357, G9000090167, G9000128763, G9000180144, Cancer Center Support Grant
OpenAlex ID: W3014938518