FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing
Year: 2020
Type: article
Abstract: Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution. By quantifying BrdU incorporation along pulse-chased replication intermediates from Saccharomyces cerevisiae, we orient 58,651 replication tracks reproducing population-based replication directionality profiles and map 4964 and 4485 individual initiation and termination events, respectively. Although most events cluster at known origins and fork merging zones, 9% and 18% of initiation and termination events, respectively, occur at many locations previously missed. Thus, FORK-seq reveals the full extent of cell-to-cell heterogeneity in DNA replication.
Source: Genome biology
Institutions: Centre National de la Recherche Scientifique, Inserm, Université Paris Cité, Université Paris Sciences et Lettres, Institut de Biologie de l'École Normale Supérieure, Epigénétique et Destin Cellulaire, Lyon 1 Université, École Normale Supérieure de Lyon, Université de Lyon, Laboratoire de Physique de l'ENS de Lyon, Laboratoire de Biologie et Modélisation de la Cellule, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Genoscope, Institut de biologie François Jacob
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,068
Awards: G1384973413, High-performance genomic profiling of DNA replication by nanopore sequencing, G1554707310, Organisation et montée en puissance d'une Infrastructure Nationale de Génomique, G2579310621, G4656594441, Dynamiques eco-évolutives des maladies infectieuses, High throughput DNA combing methods for rapid replication mapping of the human genome, G705061367, G7240651115, G7999552054, G8803503368
OpenAlex ID: W3030003218