Work

Detecting Protein–Small Molecule Interactions Using Limited Proteolysis–Mass Spectrometry (LiP-MS)

Year: 2020

Type: article

Abstract: Protein–small molecule interactions control many cellular processes, such as allosteric regulation of enzyme activity. One method to study protein–small molecule interactions in a complex sample is limited proteolysis–mass spectrometry (LiP-MS). In this approach, a small molecule is added to a complex sample such as a cell lysate, which is then briefly incubated with a nonspecific protease. Since binding of a small molecule can locally alter the protease accessibility of its target proteins, this yields condition-specific cleavage products.

Source: Trends in Biochemical Sciences

DOI: 10.1016/j.tibs.2020.05.006

Authors: Monika Pepelnjak, Natalie de Souza, Paola Picotti

Institutions: ETH Zurich, Institute for Molecular Systems Biology, University of Zurich

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,000

Funders: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, European Research Council, H2020 European Research Council