Work

Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract

Year: 2020

Type: article

Abstract: Nanoparticles have emerged as a key player in modern medicine and involved in drug delivery, it can be manufactured using various approaches including chemical, physical, and biological methods. Nanoparticles are generally characterized by their size, morphology and surface charge using advanced microscopic techniques such as SEM and TEM. This study was aimed for synthesis and characterization of silver nanoparticle and to detect their antibacterial activity using Azadirachta indica (Neem) leaves extract. The results showed a visible change of colour after the addition of Neem leaf extract to AgNO3 solution to dark brown which indicates the formation of silver nanoparticle. UV spectrophotometer showed absorbance peak in range of 390-700 nm, the result revealed the highest peak showed by concentration 1 ml at 428.80 nm. SEM and XRD analysis were used to characterize synthesize silver nanoparticles. The antibacterial activity against Gram positive Staphylococcus aureus and Gram negative Escherichia coli and Klebsiella spp Microorganisms was carried out using disc diffusion method, the results showed that the highest activity was 14 mm against S. aureus. A. indica silver nanoparticles can be used as a new nano drug in formulation and delivery. The synthesized silver nanoparticles are extremely important in various areas of life and are widely used for the benefit of human being.

Source: Science Research

DOI: 10.11648/j.sr.20200801.13