Year: 2020
Type: article
Abstract: Transient receptor potential (TRP) channel family proteins are sensors for pain, which sense variety of thermal and noxious chemicals. Sensory neurons innervating the gut abundantly express TRPA1 and TRPV1 channels and are in close proximity of gut microbes. Emerging evidence indicates a bi-directional gut-brain cross-talk in several entero-neuronal pathologies; however, the direct evidence of TRP channels interacting with gut microbial populations is lacking. Herein, we examine whether and how the knockout (KO) of TRPA1 and TRPV1 channels individually or combined TRPA1/V1 double-knockout (dKO) impacts the gut microbiome in mice. We detect distinct microbiome clusters among the three KO mouse models versus wild-type (WT) mice. All three TRP-KO models have reduced microbial diversity, harbor higher abundance of Bacteroidetes, and reduced proportion of Firmicutes. Specifically distinct arrays in the KO models are determined mainly by S24-7, Bacteroidaceae, Clostridiales, Prevotellaceae, Helicobacteriaceae, Rikenellaceae, and Ruminococcaceae. A1KO mice have lower Prevotella, Desulfovibrio, Bacteroides, Helicobacter and higher Rikenellaceae and Tenericutes; V1KO mice demonstrate higher Ruminococcaceae, Lachnospiraceae, Ruminococcus, Desulfovibrio and Mucispirillum; while A1V1dKO mice exhibit higher Bacteroidetes, Bacteroides and S24-7 and lower Firmicutes, Ruminococcaceae, Oscillospira, Lactobacillus and Sutterella abundance. Also, the abundance of taxa involved in biosynthesis of lipids and primary and secondary bile acids is higher while that of fatty acid biosynthesis-associated taxa is lower in all KO groups. To our knowledge, this is the first study demonstrating distinct gut microbiome signatures in TRPA1, V1 and dKO models and should facilitate prospective studies exploring novel diagnostic/ therapeutic modalities regarding the pathophysiology of TRP channel proteins.
Source: Pathogens
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,584
Funders: U.S. Department of Defense, North Carolina State University, National Institutes of Health, Wake Forest School of Medicine, National Institute on Aging, National Center for Advancing Translational Sciences, Wake Forest Clinical and Translational Science Institute, Wake Forest School of Medicine, Congressionally Directed Medical Research Programs
Awards: G171052541, Wake Forest Clinical and Translational Science Award, G2831378491, PROBIOTIC BIOTHERAPY TO AMELIORATE ALZHEIMER'S DISEASE., G4119534169, DEVELOPMENT OF MICROBIOME-BASED BIOMARKERS AND BIOTHERAPY FOR METFORMIN EFFICACY, Exercise Intolerance in Older HFPEF Patients, Development of Microbiome-Based Biomarkers and Biotherapy for Metformin Efficacy, G5850682332, G5883158381, G8166743225, Probiotic Biotherapy to Ameliorate Alzheimer's Disease, Wake Forest Claude D. Pepper OAIC - Renewal
OpenAlex ID: W3043846992