Work

Detection of Bio-film Production among the Most Frequent Bacterial Isolates in Cases of Chronic Suppurative Otitis Media: A Cross-sectional Study

Year: 2020

Type: article

Abstract: To detect biofilm production among the most common micro-organisms isolated from CSOM cases. Five hundred consecutive patients with a diagnosis of CSOM were enrolled in the study. Organisms were isolated using standard culture methods and biochemical reactions. Antibiotic sensitivity pattern was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Biofilm production was detected using Tube method and microtitre plate method. The most common organism isolated was Pseudomonas aeruginosa in 220 cases(44%) followed by Staphylococcus aureus in 110 cases (22%). Out of 186 isolates tested for biofilm production, 149 isolates (80%) showed biofilm production. Out of the moderately positive and strongly positive biofilm producers, 90% isolates were from unsafe CSOM. High proportion of isolates from CSOM cases shows biofilm production. Biofilm production may account for antibiotic resistance, recurrence of CSOM and failure of surgical procedures in CSOM cases.

Source: International Journal of Current Microbiology and Applied Sciences

DOI: 10.20546/ijcmas.2020.906.167

Authors: Priya Bhat, Sameer Peer, Raksha Yoganand

Institution: National Institute of Mental Health and Neurosciences

Language: English

Topic: Bacterial biofilms and quorum sensing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W3045636045