Work

Surface-Enhanced Raman Scattering Detection of Nucleic Acids Exhibiting Sterically Accessible Guanines Using Ruthenium-Polypyridyl Reagents

Year: 2020

Type: article

Abstract: Here, we report the application of surface-enhanced Raman scattering (SERS) spectroscopy as a rapid and practical tool for assessing the formation of coordinative adducts between nucleic acid guanines and ruthenium polypyridyl reagents. The technology provides a practical approach for the wash-free and quick identification of nucleic acid structures exhibiting sterically accessible guanines. This is demonstrated for the detection of a quadruplex-forming sequence present in the promoter region of the c-myc oncogene, which exhibits a nonpaired, reactive guanine at a flanking position of the G-quartets

Source: The Journal of Physical Chemistry Letters

DOI: 10.1021/acs.jpclett.0c02148

Authors: Miguel Martínez-Calvo, Luca Guerrini, Jéssica Rodríguez, Ramón A. Álvarez-Puebla, José L. Mascareñas