Work

Comparison of three different methods for the analysis of ram sperm concentration.

Year: 2020

Type: article

Abstract: Determination of sperm concentration is a critical component of semen analysis. Traditionally, the haemocytometer has been the standard tool for calibrating other techniques used to estimate sperm concentration, including photometry, Coulter counters, flow cytometry and computer-automated semen analysis (CASA). In the present study, fresh ram sperm samples (n = 7) from the Native Wallachian (NW) Slovak sheep breed were collected from one male by electro-ejaculation (EE) and analysed for sperm concentration using flow cytometer FACS Calibur, CASA Sperm VisionTM and using EVETM Automatic Cell Counter. Our results showed no significant (P ≥ 0.05) differences in the sperm concentration when analysed by these three methods. Thus, it is possible to use a cell counter to determine the approximate sperm concentration directly at the place of semen collection. This is a very practical finding since instruments such as flow cytometer or CASA are not suitable for transport to the place of semen collection.

Source: Slovak Journal of Animal Science

Authors: Andrej Baláži, Jaromír Vašíček, Andrea Svoradová, Marián Macháč, Rastislav Jurčík, J. Huba, Ivan Pavlík, Peter Chrenek

Institutions: Animal Production Research Centre, Slovak University of Agriculture in Nitra

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: diamond

Funders: Agentúra na Podporu Výskumu a Vývoja, Kultúrna a Edukacná Grantová Agentúra MŠVVaŠ SR, Vedecká Grantová Agentúra MŠVVaŠ SR a SAV

Awards: Ochrana ohrozených slovenských plemien hospodárskych zvierat v podmienkach ex situ, G4221948015, Ochrana vybraných slovenských plemien v podmienkach in vitro, G6227076073

OpenAlex ID: W3048898249

FWCI: 0.839

Cites: 22

Cited by: 5

Related to: 10